کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10604719 | 982530 | 2011 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Pectic polysaccharides from mature orange (Citrus sinensis) fruit albedo cell walls: Sequential extraction and chemical characterization
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The orange albedo alcohol insoluble solids (AIS) wall preparation, material prepared from the albedo tissue of mature orange fruit, contained 55Â mol% pectic polysaccharides, including homogalacturonan (HG), rhamnogalacturonan I (RG I) and rhamnogalacturonan II (RG II) together with Type I arabinogalactan (AG) and arabinan. It also contained cellulose (22Â mol%) and other non-cellulosic polysaccharides (14Â mol%), including xyloglucans (XGs) (10Â mol%), heteromannans (2Â mol%) and heteroxylans (2Â mol%). Sequential extraction of the AIS performed with NaOAc, CDTA, Na2CO3, 1Â M KOH and 4Â M KOH produced soluble fractions (Frs.) with different relative proportions of these polysaccharides. The NaOAc and CDTA Frs. were composed predominantly of pectic polysaccharides (89 and 97Â mol%, respectively) with only very low proportions of other non-cellulosic polysaccharides. Na2CO3 (50Â mM) released highly ramified pectic polysaccharides, whereas stronger alkali (1Â M and 4Â M KOH) liberated mostly non-cellulosic polysaccharide, comprised predominantly of XG. The NaOAc, CDTA and Na2CO3 Frs. contain low levels of 2-O-methyl-Fuc and 2-O-methyl-Xyl, suggesting the presence of RG II. To obtain the RG II for structural characterization, the NaOAc fraction was digested with a combination of endo-polygalacturonase (endo-PG; PG I and PG II) and exo-PG, and the degradation product was fractionated by anion exchange and size exclusion chromatography. The isolated product (approx. 0.5% of orange albedo AIS) contained all six diagnostic sugars and has a glycosyl linkage composition consistent with the structural model of RG II.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 84, Issue 1, 11 February 2011, Pages 484-494
Journal: Carbohydrate Polymers - Volume 84, Issue 1, 11 February 2011, Pages 484-494
نویسندگان
Indira Prabasari, Filomena Pettolino, Ming-Long Liao, Antony Bacic,