Utility of coupled-HSQC experiments in the intact structural elucidation of three complex saponins from Blighia sapida

► Non-destructive structure elucidation of complex saponins by NMR. ► Utility of 1H coupled HSQC NMR spectra for sugar identification in strongly coupled systems. ► NMR and MS characterization of saponins from Blighia sapida (ackee).