Effects of protein-simulated body fluid mixing methods on characteristics of bone-like mineral

► Bone-like minerals coated on PLGA using different methods of mixing BSA with mSBF. ► Premixing BSA with CaCl2 provides a thick mineral layer aligned to the c-axis. ► Mineral formed by premixing BSA with KH2PO4 has a low crystallinity. ► Chemical binding BSA with inorganic ions in mSBF affects crystallinity of mineral.