Incorporation of proteins and enzymes at different stages of the preparation of calcium phosphate coatings on a degradable substrate by a biomimetic methodology

Ca-P coatings, with α-amylase incorporated at the nucleation stage, showed no degradation of the film after incubation in SBF for 28 days. The release of increasing concentration of reducing sugars with degradation time revealed that α-amylase was efficiently incorporated in the coating remaining active throughout the coating preparation. This can be a strategy that will allow, in addition of conferring osteoconductive properties to biodegradable polymers, also simultaneously tailoring their degradation kinetics.