کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10737285 | 1046375 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Age-related calcium changes, oxyradical damage, caspase activation and nuclear condensation in hippocampal neurons in response to glutamate and beta-amyloid
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
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چکیده انگلیسی
Neuronal degeneration increases with age in response to stressors, but the sub-cellular mechanism is unknown, partly because of previous difficulty in studying aged neurons in isolation. We studied the mechanism of enhanced neuronal susceptibility to glutamate and β-amyloid in terms of condensed nuclei and other upstream events in hippocampal neurons cultured from old rats (24 months) compared to middle-age (10 months) and embryonic rats. Treatment of neurons from old animals with β-amyloid (or glutamate) produced condensed nuclei 1.5à (2Ã) more frequently than middle-age and 3à (4Ã) more frequently than embryonic neurons. In addition to age-related baseline levels of caspase activation, neurons from old animals showed a 50% greater increase in caspase activation compared to middle-age and embryonic neurons. In contrast to glutamate treatment, β-amyloid caused oxyradical damage as protein carbonyls increased 2-fold higher for old neurons than middle-age and 10-fold higher than embryonic neurons. Contrary to expectations, steady-state calcium levels for adult neurons did not increase in response to β-amyloid. Overall, these results suggest that aged neurons have an inherent increased susceptibility to β-amyloid toxicity through an early action of oxyradicals followed by caspase activation and nuclear condensation, a common pathway of apoptosis. Age-related glutamate toxicity involves other steps that lead to nuclear condensation, but neuron responses to calcium influx appear more important to cell death than the amount of influx.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Gerontology - Volume 40, Issue 5, May 2005, Pages 426-437
Journal: Experimental Gerontology - Volume 40, Issue 5, May 2005, Pages 426-437
نویسندگان
Gregory J. Brewer, A. Lim, Nicole G. Capps, John R. Torricelli,