Nox4- and Nox2-dependent oxidant production is required for VEGF-induced SERCA cysteine-674 S-glutathiolation and endothelial cell migration

► VEGF- or H2O2-induced EC migration was inhibited by overexpression of catalase or SOD. ► H2O2-induced S-glutathiolation of SERCA2b cysteine-674 and EC Ca2+ influx required Nox2. ► VEGF-induced S-glutathiolation of SERCA2b and EC Ca2+ influx required both Nox4 and Nox2. ► Nox4-derived H2O2 mediates VEGF-induced SERCA S-glutathiolation, Ca2+ influx, and EC migration.