Purified human paraoxonase-1 interacts with plasma membrane lipid rafts and mediates cholesterol efflux from macrophages

► Paraoxonase-1 (PON1) stimulates the capacity of HDL to mediate cholesterol efflux. ► PON1 upregulates ABCA1 gene and protein expression on J774 macrophages. ► PON1 stimulates the association of apo-AI with ABCA1 to stimulate cholesterol efflux. ► PON1 acts via an apo-AI-like mechanism to mediate cholesterol efflux.