Assessing bioenergetic function in response to oxidative stress by metabolic profiling

► The impact of oxidative stress on bioenergetics is important to assess in a cellular setting. ► Protocols are reported to obtain the bioenergetic response to oxidative stress in adherent cells. ► Oxidative phosphorylation and glycolysis are integrated to make a metabolic profile. ► Reserve capacity is the most critical parameter in cellular bioenergetics.