کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10738679 | 1046721 | 2011 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cell sensitivity to oxidative stress is influenced by ferritin autophagy
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کلمات کلیدی
SSMHBSSLMPSIHsalicylaldehyde isonicotinoyl hydrazoneTMRELysosomal membrane permeabilization - permeabilization غشای لسیوزومیRedox-active iron - آهن فعال آهن ردوکسAutophagy - اتوفاژیtetramethylrhodamine ethyl ester - اتیل استر استرهای تترامتیل رودامینOxidative stress - تنش اکسیداتیوApoptosis - خزان یاختهایFree radicals - رادیکال آزادFerritin - فریتینLysosomes - لیزوزوم هاPropidium iodide - پروتئین یدید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
To test the consequences of lysosomal degradation of differently iron-loaded ferritin molecules and to mimic ferritin autophagy under iron-overload and normal conditions, J774 cells were allowed to endocytose heavily iron loaded ferritin, probably with some adventitious iron (Fe-Ft), or iron-free apo-ferritin (apo-Ft). When cells subsequently were exposed to a bolus dose of hydrogen peroxide, apo-Ft prevented lysosomal membrane permeabilization (LMP), whereas Fe-Ft enhanced LMP. A 4-h pulse of Fe-Ft initially increased oxidative stress-mediated LMP that was reversed after another 3Â h under standard culture conditions, suggesting that lysosomal iron is rapidly exported from lysosomes, with resulting upregulation of apo-ferritin that supposedly is autophagocytosed, thereby preventing LMP by binding intralysosomal redox-active iron. The obtained data suggest that upregulation of the stress protein ferritin is a rapid adaptive mechanism that counteracts LMP and ensuing apoptosis during oxidative stress. In addition, prolonged iron starvation was found to induce apoptotic cell death that, interestingly, was preceded by LMP, suggesting that LMP is a more general phenomenon in apoptosis than so far recognized. The findings provide new insights into aging and neurodegenerative diseases that are associated with enhanced amounts of cellular iron and show that lysosomal iron loading sensitizes to oxidative stress.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 50, Issue 11, 1 June 2011, Pages 1647-1658
Journal: Free Radical Biology and Medicine - Volume 50, Issue 11, 1 June 2011, Pages 1647-1658
نویسندگان
Tino Kurz, Bertil Gustafsson, Ulf T. Brunk,