کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10738750 | 1046751 | 2009 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Selenite induces redox-dependent Bax activation and apoptosis in colorectal cancer cells
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کلمات کلیدی
NACMnTMPyPDisuccinimidyl suberateMPBBSODAPIDTTDSS4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولN-acetylcysteine - N-استیل سیستئینBax - باکسbuthionine sulfoximine - بوته یون سولفسیمیمApoptosis - خزان یاختهایdithiothreitol - دیتیوتریتولFree radicals - رادیکال آزادColorectal cancer - سرطان روده بزرگSelenium - سلنیومChemotherapy - شیمیدرمانی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Emerging evidence suggests that selenium has chemotherapeutic potential by inducing cancer cell apoptosis with minimal side effects to normal cells. However, the mechanism by which selenium induces apoptosis is not well understood. We have investigated the role of Bax, a Bcl-2 family protein and a critical regulator of the mitochondrial apoptotic pathway, in selenite-induced apoptosis in colorectal cancer cells. We found that supranutritional doses of selenite could induce typical apoptosis in colorectal cancer cells in vitro and in xenograft tumors. Selenite triggers a conformational change in Bax, as detected by the 6A7 antibody, and leads to Bax translocation into the mitochondria, where Bax forms oligomers to mediate cytochrome c release. Importantly, we show that the two conserved cysteine residues of Bax seem to be critical for sensing the intracellular ROS to initiate Bax conformational changes and subsequent apoptosis. Our results show for the first time that selenite can activate the apoptotic machinery through redox-dependent activation of Bax and further suggest that selenite could be useful in cancer therapy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 46, Issue 8, 15 April 2009, Pages 1186-1196
Journal: Free Radical Biology and Medicine - Volume 46, Issue 8, 15 April 2009, Pages 1186-1196
نویسندگان
Fang Huang, Chunlai Nie, Yang Yang, Wen Yue, Yun Ren, Yingli Shang, Xiaohui Wang, Haijing Jin, Caimin Xu, Quan Chen,