کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10738839 | 1046836 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Microdialysis studies of extracellular reactive oxygen species in skeletal muscle: Factors influencing the reduction of cytochrome c and hydroxylation of salicylate
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
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چکیده انگلیسی
Identification and quantification of specific reactive oxygen species (ROS) is essential to allow greater understanding into the role that ROS play in tissues and extracellular fluids. Previous studies have examined the reduction of cytochrome c and the hydroxylation of salicylate to detect superoxide and hydroxyl activity, respectively, although the specificity of these assays has been the subject of debate. This study aimed to identify the factors influencing hydroxylation of salicylate and reduction of cytochrome c in microdialysates from skeletal muscle extracellular fluid. Mice were anesthetized and treated with either polyethylene glycol-tagged superoxide dismutase (PEG-SOD), desferrioxamine mesylate (desferal) or NG-nitro-l-arginine methyl ester (l-NAME). A further cohort of untreated mice was also studied. Microdialysis probes were placed into the gastrocnemius muscle and perfused with salicylate or cytochrome c prior to, during, and after a period of demanding electrically stimulated contractions. Microdialysates were analysed for the reduction of cytochrome c and hydroxylation of salicylate. Contractile activity was found to increase both the reduction of cytochrome c and the hydroxylation of salicylate in the microdialysates. The reduction of cytochrome c was greater in mice treated with l-NAME compared with control untreated mice and was attenuated in mice treated with PEG-SOD. The hydroxylation of salicylate was attenuated in mice treated with desferal while there was no effect of l-NAME compared with untreated mice. Data support the hypothesis that superoxide and hydroxyl radical activity are the major contributors to the reduction of cytochrome c and hydroxylation of salicylate respectively in microdialysates from skeletal muscle extracellular fluid and indicate that these ROS are increased by contractile activity in skeletal muscle extracellular fluid.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 39, Issue 11, 1 December 2005, Pages 1460-1467
Journal: Free Radical Biology and Medicine - Volume 39, Issue 11, 1 December 2005, Pages 1460-1467
نویسندگان
Graeme L. Close, Tony Ashton, Anne McArdle, Malcolm J. Jackson,