کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10738857 | 1046838 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enzyme-independent nitric oxide formation during UVA challenge of human skin: characterization, molecular sources, and mechanisms
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کلمات کلیدی
DMEMtris-(2-carboxyethyl)phosphineQCLS-nitrosothiolsAECnNOSeNOSiNOSDTNBNOSN-ethylmaleimideUVA5,5′-dithiobis(2-nitrobenzoic acid) - 5،5'-dithiobis (2-nitrobenzoic acid)Dulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده Dulbecconeuronal NOS - NOS عصبیinducible NOS - NOS قابل القاییROS - ROSendothelial NOS - اندوتلیال NOSTCEP - ساکتPhotolysis - عکسبرداریQuantum cascade laser - لیزر آبشار کوانتومیNEM - نهNitrite - نیتریتNitric oxide - نیتریک اکسیدnitric oxide synthase - نیتریک اکسید سنتازHuman skin - پوست انسانReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Many of the local UV-induced responses including erythema and edema formation, inflammation, premature aging, and immune suppression can be influenced by nitric oxide synthase (NOS)-produced NO which is known to play a pivotal role in cutaneous physiology. Besides NOS-mediated NO production, UV radiation might trigger an enzyme-independent NO formation in human skin by a mechanism comprising the decomposition of photo-reactive nitrogen oxides. Therefore, we have examined the chemical-storage forms of potential NO-generating agents, the mechanisms and kinetics of their decomposition, and their biological relevance. In normal human skin specimens we find nitrite and S-nitrosothiols (RSNO) at concentrations 25- or 360-fold higher than those found in plasma of healthy volunteers. UVA irradiation of human skin leads to high-output formation of bioactive NO due to photo-decomposition of RSNO and nitrite which represents the primary basis for NO formation during UVA exposure. Interestingly, reduced thiols strongly augment photo-decomposition of nitrite and are essential for maximal NO release. The enzyme-independent NO formation found in human skin opens a completely new field in cutaneous physiology and will extend our understanding of mechanisms contributing to skin aging, inflammation, and cancerogenesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 38, Issue 5, 1 March 2005, Pages 606-615
Journal: Free Radical Biology and Medicine - Volume 38, Issue 5, 1 March 2005, Pages 606-615
نویسندگان
Adnana N. Paunel, André Dejam, Sven Thelen, Michael Kirsch, Markus Horstjann, Putrika Gharini, Manfred Mürtz, Malte Kelm, Herbert de Groot, Victoria Kolb-Bachofen, Christoph V. Suschek,