کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10738901 1046842 2005 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A confocal microscopy study of the very early cellular response to oxidative stress induced by zinc phthalocyanine sensitization
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی سالمندی
پیش نمایش صفحه اول مقاله
A confocal microscopy study of the very early cellular response to oxidative stress induced by zinc phthalocyanine sensitization
چکیده انگلیسی
Oxidative stress has been involved in several biological and pathological processes. Reactive oxygen species have been shown to play both beneficial and deleterious roles. The present work contributes to the understanding of the very early events of cellular response to oxidative stress. Oxidative stress was produced intracellularly by light activation of zinc phthalocyanine (ZnPc) at a light dose that did not lead to apoptosis or necrosis. Phthalocyanine was photoactivated using the 647-nm laser line of a confocal microscope through the objective lens causing oxidative stress and allowing observation of the evoked phenomena at the single cell level and in real time. Mitochondria membrane potential (ΔΨm), intracellular pH, calcium concentration, and generation of reactive oxygen species (ROS) were recorded using specific vital fluorescent probes and quantified by image processing and analysis. Subcellular localization of ZnPc was also studied in order to determine the primary and intermediate ROS target.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 39, Issue 9, 1 November 2005, Pages 1119-1127
نویسندگان
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