کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10739065 | 1046858 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Endogenous DNA damage clusters in human skin, 3-D model, and cultured skin cells
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کلمات کلیدی
PBSATCCBERFBSDMEMDSB8-oxo-7,8-dihydro-2′-deoxyguanosine - 8-اکسو-7،8-دی هیدرو-2'-دگزسی گوانوزینDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoEndogenous DNA damage - آسیب DNA داخلیCho - برایbase excision repair - تعمیر پایه پایهFree radicals - رادیکال آزادfetal bovine serum - سرم جنین گاوPrimary Human Cells - سلولهای اولیه انسانdouble-strand break - شکست دو ردیفpen/strep - قلم / استرپAmerican Type Culture Collection - مجموعه فرهنگی نوع آمریکاییPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریPenicillin/streptomycin - پنیسیلین / استرپتومایسینHuman skin - پوست انسان
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
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چکیده انگلیسی
Clustered damages-two or more oxidized bases, abasic sites, or strand breaks on opposing DNA strands within a few helical turns-are formed in DNA by ionizing radiation. Clusters are difficult for cells to repair and thus pose significant challenges to genomic integrity. Although endogenous clusters were found in some permanent human cell lines, it was not known if clusters accumulated in human tissues or primary cells. Using high-sensitivity gel electrophoresis, electronic imaging, and number average length analysis, we determined endogenous cluster levels in DNA from human skin, a 3-D skin model, and primary cultured skin cells. DNA from dermis and epidermis of human skin contained extremely low levels of endogenous clusters (a few per gigabase). However, cultured skin fibroblasts and keratinocytes-whether in monolayer cultures or in 3-D model skin cultures-accumulated oxidized pyrimidine, oxidized purine, and abasic clusters. The levels of endogenous clusters were decreased by growing cells in the presence of selenium or by increasing cellular levels of Fpg protein, presumably by increasing processing of clustered damages. These results imply that the levels of endogenous clusters can be affected by the cells' external environment and their ability to deal with DNA damage.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 39, Issue 6, 15 September 2005, Pages 832-839
Journal: Free Radical Biology and Medicine - Volume 39, Issue 6, 15 September 2005, Pages 832-839
نویسندگان
Paula V. Bennett, Noelle L. Cuomo, Sunirmal Paul, Stefan T. Tafrov, Betsy M. Sutherland,