کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10739270 | 1046869 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Intracellular generation of reactive oxygen species by contracting skeletal muscle cells
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کلمات کلیدی
DCFH-DACEED-PBSdichlorofluoresceinFCSDcfDMEMDMSO - DMSODulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoROS - ROSDimethyl sulfoxide - دیمتیل سولفواکسیدSOD - سدhorse serum - سرب اسبfetal calf serum - سرم گوساله جنینSuperoxide dismutase - سوکسوکس دیسموتازDulbecco's phosphate-buffered saline - فسفات باسیل نمک DulbeccoNitric oxide - نیتریک اکسیدReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
The aim of this work was to examine the intracellular generation of reactive oxygen species in skeletal muscle cells at rest and during and following a period of contractile activity. Intracellular generation of reactive oxygen species was examined directly in skeletal muscle myotubes using 2â²,7â²-dichlorodihydrofluorescein (DCFH) as an intracellular probe. Preliminary experiments confirmed that DCFH located to the myotubes but was readily photoxidizable during repeated intracellular fluorescence measurements and strategies to minimize this were developed. The rate of oxidation of DCFH did not change significantly over 30 min in resting myotubes, but was increased by approximately 4-fold during 10 min of repetitive, electrically stimulated contractile activity. This increased rate was maintained over 10 min following the end of the contraction protocol. DCF fluorescence was distributed evenly throughout the mytotube with no evidence of accumulation at any specific intracellular sites or localization to mitochondria. The rise in DCF fluorescence was effectively abolished by treatment of the myotubes with the intracellular superoxide scavenger, Tiron. Thus these data appear to represent the first direct demonstration of a rise in intracellular oxidant activity during contractile activity in skeletal muscle myotubes and indicate that superoxide, generated from intracellular sites, is the ultimate source of oxidant(s) responsible for the DCFH oxidation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 39, Issue 5, 1 September 2005, Pages 651-657
Journal: Free Radical Biology and Medicine - Volume 39, Issue 5, 1 September 2005, Pages 651-657
نویسندگان
Francis McArdle, David M. Pattwell, Aphrodite Vasilaki, Anne McArdle, Malcolm J. Jackson,