کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10739589 1046881 2005 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Exchange of 18O in the reaction of peroxynitrite with CO2
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی سالمندی
پیش نمایش صفحه اول مقاله
Exchange of 18O in the reaction of peroxynitrite with CO2
چکیده انگلیسی
We have observed an exchange of 18O in the reactions of CO2 with peroxynitrite using membrane-inlet mass spectrometry and HPLC negative electrospray ionization mass spectrometry. The exchange appeared on addition of peroxynitrite to a solution containing 18O-labeled CO2 in equilibrium with bicarbonate. It was observed as a temporarily enhanced rate of depletion of 18O from CO2, a rate that was greater than the rate of 18O depletion caused by the hydration/dehydration cycle of CO2. In addition, we detected the appearance of mass peaks attributed to 18O in product NO3-.As a further measure of the 18O exchange, there was a redistribution of 18O such that the ratio of doubly to singly labeled CO2 could not be described by the binomial expansion. This is not due to the hydration/dehydration cycle of CO2 but most likely to recycling of CO2 in the reaction with peroxynitrite. This 18O exchange associated with the reactions of CO2 and peroxynitrite may open a new methodology for studying this significant process.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 38, Issue 1, 1 January 2005, Pages 93-97
نویسندگان
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