Molecular mechanisms of fMLP-induced superoxide generation and degranulation in mouse neutrophils

In this manuscript, involvement of PLD in fMLP-induced superoxide generation and degranulation were re-investigated using PLD−/− neutrophils, and the molecular mechanisms of these neutrophil functions were examined. Neither PLD1 nor PLD2 is involved in these fMLP-induced neutrophil functions. The results obtained in this study provide evidence that cPKC plays an important role in fMLP-induced superoxide generation. On the other hand, Ca2+-dependent signaling pathway and cPKC seem to be involved in degranulation.