کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10795748 | 1052593 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The Saccharomyces cerevisiae gene YPR011c encodes a mitochondrial transporter of adenosine 5â²-phosphosulfate and 3â²-phospho-adenosine 5â²-phosphosulfate
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کلمات کلیدی
MCFCOAPAPThermotoleranceAPs - AP هاPAPs - PAP هاadenosine 3′,5′-diphosphate - آدنزین 3 '، 5'-دی فسفاتadenosine 5′-phosphosulfate - آدنوزین 5'-فسفسولفاتMitochondrial carrier - حامل میکاتونیTransporter - حمل کنندهMitochondrial carrier family - خانواده حامل های میتوکندریSaccharomyces cerevisiae - ساکارومایسس سرویزیهMitochondrion - میتوکندریونcoenzyme A - کوآنزیم A
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The Saccharomyces cerevisiae gene YPR011c encodes a mitochondrial transporter of adenosine 5â²-phosphosulfate and 3â²-phospho-adenosine 5â²-phosphosulfate The Saccharomyces cerevisiae gene YPR011c encodes a mitochondrial transporter of adenosine 5â²-phosphosulfate and 3â²-phospho-adenosine 5â²-phosphosulfate](/preview/png/10795748.png)
چکیده انگلیسی
The genome of Saccharomyces cerevisiae contains 35 members of the mitochondrial carrier family, nearly all of which have been functionally characterized. In this study, the identification of the mitochondrial carrier for adenosine 5â²-phosphosulfate (APS) is described. The corresponding gene (YPR011c) was overexpressed in bacteria. The purified protein was reconstituted into phospholipid vesicles and its transport properties and kinetic parameters were characterized. It transported APS, 3â²-phospho-adenosine 5â²-phosphosulfate, sulfate and phosphate almost exclusively by a counter-exchange mechanism. Transport was saturable and inhibited by bongkrekic acid and other inhibitors. To investigate the physiological significance of this carrier in S. cerevisiae, mutants were subjected to thermal shock at 45 °C in the presence of sulfate and in the absence of methionine. At 45 °C cells lacking YPR011c, engineered cells (in which APS is produced only in mitochondria) and more so the latter cells, in which the exit of mitochondrial APS is prevented by the absence of YPR011cp, were less thermotolerant. Moreover, at the same temperature all these cells contained less methionine and total glutathione than wild-type cells. Our results show that S. cerevisiae mitochondria are equipped with a transporter for APS and that YPR011cp-mediated mitochondrial transport of APS occurs in S. cerevisiae under thermal stress conditions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1837, Issue 2, February 2014, Pages 326-334
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1837, Issue 2, February 2014, Pages 326-334
نویسندگان
Simona Todisco, Maria Antonietta Di Noia, Alessandra Castegna, Francesco Massimo Lasorsa, Eleonora Paradies, Ferdinando Palmieri,