کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10795762 | 1052594 | 2014 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
ROS generation and multiple forms of mammalian mitochondrial glycerol-3-phosphate dehydrogenase
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کلمات کلیدی
HRPHQNOCoQ2-n-heptyl-4-hydroxyquinoline N-oxideantimycin ABATSupercomplexOXPHOSSDHDCPIPFeCNmGPDH2,6-dichlorophenolindophenol - 2،6-dichlorophenolindophenolROS - ROSsn-glycerol-3-phosphate - اسپی گلیسرول 3 فسفاتHAR - اندbrown adipose tissue - بافت چربی قهوه ایROS production - تولید ROSsuccinate dehydrogenase - سوکسیناد دهیدروژنازOxidative phosphorylation - فسفوریلاسیون اکسیداتیوMyxothiazol - میکسوتیازولHorseradish peroxidase - پراکسیداز هوررادیشCoenzyme Q - کوآنزیم Qmitochondrial glycerol-3-phosphate dehydrogenase - گلیسرول 3-فسفات دهیدروژناز میتوکندریReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Overproduction of reactive oxygen species (ROS) has been implicated in a range of pathologies. Mitochondrial flavin dehydrogenases glycerol-3-phosphate dehydrogenase (mGPDH) and succinate dehydrogenase (SDH) represent important ROS source, but the mechanism of electron leak is still poorly understood. To investigate the ROS production by the isolated dehydrogenases, we used brown adipose tissue mitochondria solubilized by digitonin as a model. Enzyme activity measurements and hydrogen peroxide production studies by Amplex Red fluorescence, and luminol luminescence in combination with oxygraphy revealed flavin as the most likely source of electron leak in SDH under in vivo conditions, while we propose coenzyme Q as the site of ROS production in the case of mGPDH. Distinct mechanism of ROS production by the two dehydrogenases is also apparent from induction of ROS generation by ferricyanide which is unique for mGPDH. Furthermore, using native electrophoretic systems, we demonstrated that mGPDH associates into homooligomers as well as high molecular weight supercomplexes, which represent native forms of mGPDH in the membrane. By this approach, we also directly demonstrated that isolated mGPDH itself as well as its supramolecular assemblies are all capable of ROS production.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1837, Issue 1, January 2014, Pages 98-111
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1837, Issue 1, January 2014, Pages 98-111
نویسندگان
TomáÅ¡ MráÄek, EliÅ¡ka Holzerová, ZdenÄk Drahota, Nikola KováÅová, Marek Vrbacký, Pavel JeÅ¡ina, Josef HouÅ¡tÄk,