Iron/sulfur proteins biogenesis in prokaryotes: Formation, regulation and diversity

► Fe/S assembly understanding was enhanced by structural analysis of IscU-IscS complex. ► A-type carriers allow for the delivery of preformed clusters. ► Fe/S homeostasis is controlled by IscR, Fur and RyhB. ► Metal toxicity is mediated via Fe/S proteins. ► Fe/S biosynthesis systems show diversity in both genetic organization and factors content.