Mechanosensitive activation of CFTR by increased cell volume and hydrostatic pressure but not shear stress

Increased cell volume induced by intracellular injection of different volumes increases the activity of human CFTR expressed in Xenopus oocytes. A) Representative recording observing activation of CFTR initiated by forskolin and IBMX application and the subsequent injection of 55 nl intracellular analogous solution. B) Activation by injection was volume dependent.128