Actin filaments attachment at the plasma membrane in live cells cause the formation of ordered lipid domains

► Ordered plasma membrane domains exist in resting cells. ► Ordered membrane domains form when actin filaments attach to the plasma membrane. ► Phosphoinositides link actin dynamics to plasma membrane order. ► Intracellular actin dynamics are reflected in the outer leaflet of the plasma membrane. ► Molecular patching induces actin polymerisation and ordered domain formation.