Grazing Incidence X-ray Diffraction and Brewster Angle Microscopy studies on domain formation in phosphatidylethanolamine/cholesterol monolayers imitating the inner layer of human erythrocyte membrane

► Molecular organization of artificial inner layer of human erythrocyte membrane ► Cholesterol concentration affects the morphology of phosphatidylethanolamine film. ► Cholesterol-poor and cholesterol-rich domains coexist in model membrane. ► Domain composition is independent of sterol concentration in the mixed monolayer.