Reorganization of lipid domain distribution in giant unilamellar vesicles upon immobilization with different membrane tethers

► Different membrane tethers present distinct membrane phase partition properties. ► Some membrane tethers drastically changed domain distribution of immobilized GUVs. ► Protein surfaces tethered to the membrane stabilize membrane lipid domains. ► Domain stabilization in the tethered surface is due to phase segregation of tether.