Synergistic action of Galleria mellonella anionic peptide 2 and lysozyme against Gram-negative bacteria

► G. mellonella lysozyme caused alterations of Gram-positive bacteria cell surface. ► G. mellonella anionic peptide 2 increased E. coli membrane perforation by lysozyme. ► G. mellonella anionic peptide 2 did not influence lysozyme muramidase activity. ► G. mellonella anionic peptide 2 decreased a turgor pressure of the bacterial cells. ► E. coli treatment by Gm lysozyme and anionic peptide2 induced NaCl crystallization.