Interfacial behavior and structural properties of a clinical lung surfactant from porcine source

► Surfacen is a cholesterol-depleted clinical pulmonary surfactant of porcine origin. ► Interfacial adsorption, compressibility and stability of Surfacen mimic those of native surfactant. ► Surfacen films exhibit a particularly marked and stable segregation of ordered phases. ► A network of interconnected nanostructures sustains the surface properties of Surfacen.