A modified squeeze-out mechanism for generating high surface pressures with pulmonary surfactant

► Direct evidence for a modified squeeze-out mechanism for pulmonary surfactant. ► AFM height images showing monolayer-multilayer transition and progression of multilayer structures. ► Multilayer structures form in LE phase and at edge of LC domains. ► ToF-SIMS data showing unsaturated PL in multilayer structures at high surface pressure. ► ToF-SIMS data showing saturated PL in remaining monolayer at high surface pressure.