Na,K-ATPase activity modulates Src activation: A role for ATP/ADP ratio

►Characterization of the Na,K-ATPase signaling complex in vitro. ►Inhibition of Na,K-ATPase activity increases levels of phosphorylated Src protein. ►High ATP and low ADP concentrations are required for phosphorylation of Src. ►Na,K-ATPase activity and Src phosphorylation are linked to each other in signaling.