The human OCTN1 (SLC22A4) reconstituted in liposomes catalyzes acetylcholine transport which is defective in the mutant L503F associated to the Crohn's disease

► hOCTN1 transporter reconstituted in liposomes catalyzes acetylcholine transport. ► The transporter in proteoliposome has the same orientation as in cell membrane. ► Transport occurs as uptake or efflux from proteoliposomes (cells). ► Efflux is physiologically related to the non-neuronal acetylcholine system. ► Acetylcholine efflux is defective in the Crohn's disease associated L503F mutant.