Characterization of daptomycin oligomerization with perylene excimer fluorescence: Stoichiometric binding of phosphatidylglycerol triggers oligomer formation

► Daptomycin was labeled with perylene to produce excimer fluorescence. ► Excimer fluorescence on liposomes and bacterial cells prove oligomerization. ► Oligomerization is triggered by stoichiometric quantities of phosphatidylglycerol.