Comparative NMR analysis of an 80-residue G protein-coupled receptor fragment in two membrane mimetic environments

► NMR analysis of a double transmembrane fragment of a GPCR, Ste2p(G31-T110). ► Ste2p(G31-T110) in TFE:water (1:1) resulted in a transiently folded helical hairpin. ► Comparison of NMR structures of Ste2p(G31-T110) in micelles and TFE:water. ► Chemical shift correlation analysis of shifts derived in two membrane mimetics.