The effect of oxycholesterols on thermo-induced membrane dynamics

► We demonstrate that oxy-cholesterol-containing lipid vesicles were more sensitive to temperature. ► We show that keto functional group rendered lipid vesicles more thermo-sensitive than OH group at carbon 7. ► We show that OH group at C 25 has the same effect as the keto group at C 7. ► Characterization of the membrane dynamics provide a new insight into oxidative-stressed membranes. ► We show that temperature change in conjunction with membrane oxidation induces membrane instability.