Desmosterol replaces cholesterol for ligand binding function of the serotonin1A receptor in solubilized hippocampal membranes: Support for nonannular binding sites for cholesterol?

► Desmosterol restores ligand binding function of solubilized serotonin1A receptors. ► Nonannular binding of cholesterol is crucial for the serotonin1A receptor function. ► Caution should be exercised while interpreting results of cholesterol manipulation.