Electromediated formation of DNA complexes with cell membranes and its consequences for gene delivery

► DNA/membrane interaction occurs during cell electrotransfection. ► This interaction is imaged at unprecedent time resolution (ms). ► Observations are consistent with our theoretical model. ► DNA aggregates are formed in the vicinity of or in the plasma membrane. ► Successive electric pulses always lead to accumulation of DNA exactly at the same location than after the first pulse.