Functional analysis of the conserved hydrophobic gate region of the magnesium transporter CorA

► Mutational study of the ion gate of Mg2+ transporter CorA. ► Effect of exchange of Leu294 with a positively charged residue on gating of CorA (Leu294Arg mutant). ► Effect of exchange of Leu294 with a negatively charged residue on gating of CorA (Leu294Asp mutant). ► Effect of removal of side chain at position 294 on gating of CorA (Leu294Gly mutant).