Monitoring glycolipid transfer protein activity and membrane interaction with the surface plasmon resonance technique

►Surface plasmon resonance is a useful technique to study lipid transfer proteins. ►Tryptophan 96 is crucial for the transfer activity of glycolipid transfer protein. ►Tryptophan 142 is important for the membrane interaction of GLTP. ►The membrane environment of the substrate affects transfer mediated by GLTP. ►GLTP prefers to interact with more tightly packed membranes.