Impact of the biofilm mode of growth on the inner membrane phospholipid composition and lipid domains in Pseudomonas aeruginosa

►Pseudomonas aeruginosa lipidome is affected by biofilm growth mode. ►Biofilm growth mode leads to a decrease of the uneven-numbered chain phospholipids. ►Accumulation of long chain lipids is also observed. ►Reconstituted lipid monolayers show larger condensed domains with sessile bacteria.