α-Hemolysin pore formation into a supported phospholipid bilayer using cell-free expression

►The toxin α-hemolysin-eGFP is synthesized in a cell-free system. ►The toxin is expressed in vesicles and on a supported phospholipid bilayer. ►α-hemolysin-eGFP pore formation is studied with a quartz crystal microbalance. ►Adsorption kinetics of the toxin onto the membrane is described. ►Pores are visualized by atomic force microscopy.