The prediction and characterization of YshA, an unknown outer-membrane protein from Salmonella typhimurium

► Outer-membrane protein genomics and proteomics of Salmonella typhimurium. ► Ysha/OmpL a conserved, putative outer-membrane protein of unknown function. ► Biophysical characterization demonstrates refolding in detergents and bilayers. ► Outer-membrane protein identification algorithm is validated.