کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10798076 | 1053276 | 2007 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Peptide degradation is a critical determinant for cell-penetrating peptide uptake
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کلمات کلیدی
PBSCPPPTDCho - برایChinese hamster ovarian - تخمدان هامستر چینیDegradation - تنزلUptake - جذبextracellular - خارج سلولیIntracellular - داخل سلولیProtein transduction domain - دامنه انتقال پروتئینProtein transduction domains - دامنه های انتقال پروتئینPhosphate buffered saline - فسفات بافر شورmap - نقشهCell-penetrating peptide - پپتید نفوذ سلولی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Peptide degradation is a critical determinant for cell-penetrating peptide uptake Peptide degradation is a critical determinant for cell-penetrating peptide uptake](/preview/png/10798076.png)
چکیده انگلیسی
Cell-penetrating peptide mediated uptake of labels appears to follow an equilibrium-like process. However, this assumption is only valid if the peptides are stabile. Hence, in this study we investigate intracellular and extracellular peptide degradation kinetics of two fluorescein labeled cell-penetrating peptides, namely MAP and penetratin, in Chinese hamster ovarian cells. The degradation and uptake kinetics were assessed by RP-HPLC equipped with a fluorescence detector. We show that MAP and penetratin are rapidly degraded both extracellularly and intracellularly giving rise to several degradation products. Kinetics indicates that intracellularly, the peptides exist in (at least) two distinct pools: one that is immediately degraded and one that is stabile. Moreover, the degradation could be decreased by treating the peptides with BSA and phenanthroline and the uptake was significantly reduced by cytochalasin B, chloroquine and energy depletion. The results indicate that the extracellular degradation determines the intracellular peptide concentration in this system and therefore the stability of cell-penetrating peptides needs to be evaluated.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1768, Issue 7, July 2007, Pages 1769-1776
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1768, Issue 7, July 2007, Pages 1769-1776
نویسندگان
Caroline Palm, Mala Jayamanne, Marcus Kjellander, Mattias Hällbrink,