کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10798131 | 1053298 | 2005 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of CLCA protein expressed in ductal cells of rat salivary glands
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
A molecular entity for Ca2+-dependent Clâ transport has not been well characterized in salivary cells. Here, we identify a rat CLCA homologue (rCLCA1) using a polymerase chain reaction (PCR)-based strategy. The full length of the isoform was 3.3 kb, and the predicted open reading frame encoded a 903-amino acid protein. Immunoblotting using a specific anti-rCLCA antibody recognizing near the amino-terminus showed the expression of N-glycosylated 120- and 86-kDa proteins in the membrane fraction of rCLCA1-transfected HEK293 cells. Reverse transcription-PCR results showed mRNA expressions in rat submandibular gland (SMG), ileum, and lung. Intense immunostaining was detected in the striated ducts, but not in the acinar cells, of SMG. Immunoblot for the membrane fraction of SMG revealed the existence of 137- and 90-kDa protein species. N-glycosidase F reduced the size of these bands toward those of the deglycosylated forms in the transfected HEK293 cells. A marked ionomycin-induced Clâ conductance was observed in the transfected cells. The current was Ca2+-dependent and sensitive to niflumic acid and DIDS. rCLCA1 proteins are probably responsible for modulation of Ca2+-dependent Clâ transport in salivary ductal cells, where the 137- and 90-kDa proteins may be modified posttranslationally in a manner similar to those in the heterologous expression system.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1715, Issue 2, 15 September 2005, Pages 132-144
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1715, Issue 2, 15 September 2005, Pages 132-144
نویسندگان
Jun Yamazaki, Kazuhiko Okamura, Kazunari Ishibashi, Kenji Kitamura,