کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10798144 | 1053300 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Two-color fluorescent probes for imaging the dipole potential of cell plasma membranes
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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![عکس صفحه اول مقاله: Two-color fluorescent probes for imaging the dipole potential of cell plasma membranes Two-color fluorescent probes for imaging the dipole potential of cell plasma membranes](/preview/png/10798144.png)
چکیده انگلیسی
The dipole potential (Ψd) constitutes a large and functionally important part of the electrostatic potential of cell plasma membranes. However, its direct measurement is not possible. Herein, new 3-hydroxyflavone fluorescent probes were developed that respond strongly to Ψd changes by a variation of the intensity ratio of their two well-separated fluorescence bands. Using fluorescence spectroscopy with cell suspensions and confocal microscopy with adherent cells, we showed, for the first time, two-color fluorescence ratiometric measurement and visualization of Ψd in cell plasma membranes. Using this new tool, a heterogeneous distribution of this potential within the membrane was evidenced.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1712, Issue 2, 1 July 2005, Pages 128-136
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1712, Issue 2, 1 July 2005, Pages 128-136
نویسندگان
Vasyl V. Shynkar, Andrey S. Klymchenko, Guy Duportail, Alexander P. Demchenko, Yves Mély,