کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10798231 | 1053308 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Formation of supported phospholipid bilayers via co-adsorption with β-d-dodecyl maltoside
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We have investigated the formation of supported model membranes via the adsorption of phospholipid-surfactant mixtures at the Si-water interface by specular neutron reflection. The adsorption of mixed micelles of the nonionic surfactant β-d-dodecyl maltoside and DOPC or POPC was determined as a function of bulk concentration, and using d25-β-d-dodecyl maltoside, the composition of DOPC and POPC bilayers was determined. Bilayer thicknesses of 39±3 Ã
for DOPC and 41±3 Ã
for POPC agree well with data from bulk lamellar phases for both lipids, and the average area per lipid molecule can be varied from 62 to 115 Ã
2 by varying the bulk concentrations used. The amount of surfactant in the bilayer is very sensitive to the bulk volume-to-surface area ratio, but it can be fully eliminated by ensuring a sufficiently large dilution/rinsing volume of the solution.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1668, Issue 1, 1 February 2005, Pages 17-24
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1668, Issue 1, 1 February 2005, Pages 17-24
نویسندگان
Hanna P. Vacklin, F. Tiberg, R.K. Thomas,