کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10799016 | 1054221 | 2015 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular contacts of ribose-phosphate backbone of mRNA with human ribosome
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Molecular contacts of ribose-phosphate backbone of mRNA with human ribosome Molecular contacts of ribose-phosphate backbone of mRNA with human ribosome](/preview/png/10799016.png)
چکیده انگلیسی
In this work, intimate contacts of riboses of mRNA stretch from nucleotides in positions +Â 3 to +Â 12 with respect to the first nucleotide of the P site codon were studied using cross-linking of short mRNA analogs with oxidized 3â²-terminal riboses bound to human ribosomes in the complexes stabilized by codon-anticodon interactions and in the binary complexes. It was shown that in all types of complexes cross-links of the mRNA analogs to ribosomal protein (rp) uS3 occur and the yield of these cross-links does not depend on the presence of tRNA and on sequences of the mRNA analogs. Site of the mRNA analogs cross-linking in rp uS3 was mapped to the peptide in positions 55-64 that is located away from the mRNA binding site. Additionally, in complexes with P site-bound tRNA, riboses of mRNA nucleotides in positions +Â 4 to +Â 7 cross-linked to the C-terminal tail of rp uS19 displaying a contact specific to the decoding site of the mammalian ribosome, and tRNA bound at the A site completely blocked this cross-linking. Remarkably, rps uS3 and uS19 were also able to cross-link to the fragment of HCV IRES containing unstructured 3â²-terminal part restricted by the AUGC tetraplet with oxidized 3â²-terminal ribose. However, no cross-linking to rp uS3 was observed in the 48S preinitiation complex assembled in reticulocyte lysate with this HCV IRES derivative. The results obtained show an ability of rp uS3 to interact with single-stranded RNAs. Possible roles of rp uS3 region 55-64 in the functioning of ribosomes are discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms - Volume 1849, Issue 8, August 2015, Pages 930-939
Journal: Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms - Volume 1849, Issue 8, August 2015, Pages 930-939
نویسندگان
Dmitri E. Sharifulin, Anastasia S. Grosheva, Yulia S. Bartuli, Alexey A. Malygin, Maria I. Meschaninova, Aliya G. Ven'yaminova, Joachim Stahl, Dmitri M. Graifer, Galina G. Karpova,