Functions of the DExD/H-box proteins in nuclear pre-mRNA splicing

► Spliceosome performs nuclear pre-mRNA splicing so as to produce functional mRNA. ► DExD/H-box proteins open specific spliceosomal RNA duplexes and/or remove proteins. ► As a result, DExD/H-box proteins help to shape the spliceosomal catalytic center. ► Functions of DExD/H-box splicing factors and the outlook of the field are reviewed.