Genome-wide studies of mRNA synthesis and degradation in eukaryotes

► Complementary methodologies allow discrimination among genome-wide transcription features. ► Comparing nascent and mature TR should detect post-transcriptional differences. ► Intragenic distribution of RNA pol II is influenced by nucleosome positioning. ► RNA pol dynamics results from the interplay with chromatin and elongation factors. ► A model: transcriptional and post-transcriptional regulons would be interconnected.