کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10799354 | 1054318 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
MAPK pathway contributes to density- and hypoxia-induced expression of the tumor-associated carbonic anhydrase IX
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کلمات کلیدی
PI3KHIFCA IXCA9MAPK - MAPKCell density - تراکم سلولTranscriptional regulation - تنظیم ترانزیتیHypoxia Inducible Factor - فاکتور قابل تشخیص هیپوکسیphosphatidylinositol-3-kinase - فسفاتیدیلینواستیل-3-کینازPromoter activity - فعالیت تبلیغاتیMAPK pathway - مسیر MAPKHypoxia - هیپوکسیCarbonic anhydrase IX - کربنیک آن هیدراز IXmitogen-activated kinase - کیناز فعال با mitogen
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Transcription of the CA9 gene coding for a tumor-associated carbonic anhydrase IX (CA IX) isoform is regulated by hypoxia via the hypoxia-inducible factor 1 (HIF-1) and by high cell density via the phosphatidylinositol-3-kinase (PI3K) pathway. We examined the role of the mitogen-activated protein kinase (MAPK) pathway in the control of CA9 gene expression. Inhibition of MAPK signaling by U0126 in HeLa cells led to reduced activity of the PR1-HRE-luc CA9 promoter construct and decreased CA IX protein levels in dense culture as well as in hypoxia. Similar reduction was obtained by expression of a dominant-negative ERK1 mutant and was also observed in U0126-treated HIF-1α-deficient Ka13 cells. Simultaneous treatment with the MAPK and PI3K inhibitors U0126 and LY 294002 had stronger effect than individual inhibition of these pathways. Taken together, our results suggest that besides the PI3K pathway, the MAPK cascade is involved in the regulation of CA9 gene expression under both hypoxia and high cell density.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression - Volume 1729, Issue 1, 25 May 2005, Pages 41-49
Journal: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression - Volume 1729, Issue 1, 25 May 2005, Pages 41-49
نویسندگان
Juraj Kopacek, Monika Barathova, Franck Dequiedt, Janka Sepelakova, Richard Kettmann, Jaromir Pastorek, Silvia Pastorekova,