کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10799373 | 1054324 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transcriptional regulation of FKLF-2 (KLF13) gene in erythroid cells
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Transcriptional regulation of FKLF-2 (KLF13) gene in erythroid cells Transcriptional regulation of FKLF-2 (KLF13) gene in erythroid cells](/preview/png/10799373.png)
چکیده انگلیسی
FKLF-2 (KLF13) was cloned from fetal globin-expressing tissues and has been shown to be abundantly expressed in erythroid cells. In this study we examined the transcriptional regulation of the KLF13 gene. A 5.5 kb 5â² flanking region cloned from mouse erythroleukemia (MEL) cell genomic DNA showed that major cis regulatory activities exist in the 550 bp sequence to the unique transcription start site, and that the promoter is more active in K562 cells than in COS-7 cells. The promoter was trans-activated by co-expressed GATA-1 through the sequence containing two CCAAT motifs, suggesting that GATA-1 is involved in the abundant expression of KLF13 mRNA in the erythroid tissue. Dual action, i.e. activating effect in COS-7 and repressive effect in K562 cell, was observed on its own promoter, suggesting a feedback mechanism for the transcriptional control of the KLF13 gene in the erythroid environment. These findings provide an insight on the mechanism of inducible mRNA expression of the KLF13 gene in erythroid cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression - Volume 1727, Issue 2, 14 February 2005, Pages 125-133
Journal: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression - Volume 1727, Issue 2, 14 February 2005, Pages 125-133
نویسندگان
Ayako Mitsuma, Haruhiko Asano, Tomohiro Kinoshita, Takashi Murate, Hidehiko Saito, George Stamatoyannopoulos, Tomoki Naoe,