Detection and identification of cancerous murine fibroblasts, transformed by murine sarcoma virus in culture, using Raman spectroscopy and advanced statistical methods

► Raman spectroscopy may be used to detect malignant cells transformed by MuSV. ► 80-85% success was achieved in classifying the control NIH/3T3 and NIH-MuSV cells. ► Measurements from membranes' region sites are more effective for differentiation. ► Membrane changes caused by malignancy may contribute to classification procedure.