PKA and cAMP stimulate proliferation of mouse embryonic stem cells by elevating GLUT1 expression mediated by the NF-κB and CREB/CBP signaling pathways

► This study focused on PKA-mediated modulation of glucose uptake and proliferation. ► 6-Phenyl cAMP increased 2-DG uptake and GLUT1 translocation to the plasma membrane. ► Expression of GLUT1 was regulated by CREB/CBP/TORC2 complex formation through NF-κB. ► PKA enhanced the cell proliferation through GLUT1 expression and 2-DG uptake.